1. The model plant for biotechnological research is
A. Tomato B. Rice C. Arabidopsis D. Corn
2. The Ti and Ri plasmids are found in the following bacteria
A. Bacillus B. Rhizobium C. Agrobacterium D. Azospirullum
3. The concept of totipotency was proposed by
A. Nobecourt B. Gottlieb Haberlandt C. White D. Miller
4. Generation of haploid plants from microspores was first demonstrated by
A. Cocking B. Guha and Maheswari C. White and Went D. Skoog
5. The enzymes used in cutting DNA into smaller fragments are
A. Methylase B. Ligase C. Restriction enzymes D. Polymerase
6. Reverse transcriptase enzymes is used in
A. cDNA synthesis B. RNA synthesis C. Vector synthesis D. Virus synthesis
7. Electroporation is a technique of
A. Gene isolation B. Gene alteration C. Gene transfer
D. Gene identification
8. Blue/ white selection method of identifying transformed colonies is based on the presence of a gene called
A. Lac A B. Lac D C. Lac X D. Lac Z
9. The bond that joins amino acids is
A. Disulphide bond B. Carboxyl bond C. Peptide bond
D. Glyceride bond
10. The enzyme used to join two heterologous DNA molecules from two different sources is
A. Protease B. Polymerase C. Helicase D. Ligase
11. The term ‘Biotechnology’ was coined by
A. Charles Dickson B. Darwin C. Karl Ereky D. Southern
12. The morphogenesis under *in-vitro* depends on the balance between
A. Auxin-Gibberllines - B. Auxin-Cytokinins C. Auxin-ABA
D. Auxin-Vitamins
13. The Barnase and Bar star male sterile gene used in brassica was isolated from
A. Agrobacterium rhizogeneous
B. Bacillus thuringiensis
C. Bacillus amyloliquefaciens
D. Bacillus subtilis
14. A synthetic variety may be an advanced generation of a
A. Single cross
B. Top cross
C. Polycross
D. Populations
15. Which of the following techniques can be utilized most efficiently for map based gene cloning?
A. Chromosome banding
B. Chromosome landing
C. Chromosome painting
D. Chromosome walking
16. Among different methods of diallele analyses proposed by Griffings (1956), which one is the most suited for practical plant breeding?
A. Parents and F1’s
B. F1’s and reciprocals
C. F1’s only
D. Parents, F1’s and reciprocals
17. When selection favours one of the two extreme types of individuals in a population, it is referred to as
A. Disruptive selection
B. Stabilized selection
C. Natural selection
D. Directional selection
18. If additive gene action is most important for a trait, the best and simplest basis of selection
A. Pedigree selection
B. Progeny selection
C. Individual selection
D. Mass selection
19. Blast x searches used for
A. Translated nucleotide database using a protein query
B. Protein data base using a nucleotide query
C. Protein data base using a protein query
D. Nucleotide database using a nucleotide query
20. Northern blotting can be used to analyze samples of
A. DNA
B. proteins
C. RNA
D. DNA and RNA
21. In TILLING experiments most commonly used mutagen are
A. UV rays
B. Gamma rays
C. X-rays
D. EMS
22. Orthologs are defined as:
A. Homologous sequences in different species that arose from a common ancestral gene during speciation
B. Homologous sequences that share little amino acid identity but share great structural similarity
C. Homologous sequences in the same species that arose through gene duplication
D. Homologous sequences in the same species which have similar and often redundant functions
23. Which one of the anonymous DNA marker system could be regarded as single locus, multiallelic and co-dominant marker?
A. RAPD
B. SSR
C. SNP
D. RFLP
24. Which among the following is a multi-parent mapping population?
A. RILs
B. NILS
C.MAGIC
D. F2-3
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