1: History of plant tissue culture and genetic engineering; scope and
importance in crop improvement
1 …………………… known as father of plant tissue culture.
(a) Haberlandt (b) Robbins
(c) White (d) Miller
2 The hormone 'Kinetin' was discovered by
(a) Melchers et al. (b) Miller et al.
(c) Power (d) Braun
3 The meristem culture technique is developed by
(a) Morel and Martin (b) Murashige
(c) Webber (d) Redenbergh
4 Karl Ereky basically which of the following
(a) Hungarian Engineering
(b) Italian scientist
(c) Hungarian Biochemist (d) Hungarian Biotechnologist
5 Biotechnology is the _________use of biological agent
(a) Open (b) Controlled
(c) Accessible (d) Limited
6 The term totipotency was coined by
(a) Morgan (b) Volchting
(c) Tulecke (d) White
7 Van Overbeek (1941) was used which natural plant product for
growth of immature embryo
(a) Coconut milk (b) Liquid endosperm
(c) Plant endosperm (d) Both A and B
8 First haploid plants were produced from pollen grain of datura by
(a) Power et al Guha and Maheshwari
(c) Guha Maheshwari
9 The first embryo culture were developed by …..
(a) Tracul (b) White
(c) Henning (d) Robbin
10 The term somaclonal variation was introduced by
(a) Takebe (b) Larkin
(c) Larkin and Scowcroft (d) Scowcroft
11 Who is regarded as father of Indian embryology
(a) Guha (b) Maheshawari
(c) Guha and Maheshwari (d) Bourgin and Nitsch
12 Who were first time used commercial enzyme for protoplast isolation
(a) Takebe et al
(b) Cocking et al
(c) White (d) Both A and B
13 In which of the year Maxam-Gilbert gave technology for DNA
sequencing?
(a) 1977 (b) 1987
(c) 1967 (d) 1957
14 Biolistic gene transformation method for plant transformation was
developed by
(a) Borton (b) Klien
(c) Williams (d) Smith
15 Biolistic gene transformation method was developed in year……..
(a) 1987 (b) 1990
(c) 1995 (d) 2000
16 In which year International Rice Genome Sequencing Project was
completed?
(a) 2000 (b) 2005
(c) 2010 (d) 2015
17 The term biotechnology was coined by
(a) Kary Mullis (b) Volchting
(c) Webber (d) Karl Ereky
18 Bt gene isolated by
(a) Barton et al (b) Fodor
(c) Williams (d) Powell
19 Biotechnology is the _________use of biological agent
(a) Open (b) Controlled
(c) Accessible (d) Limited
20 The chemical used to protect cell or tissue against freezing injury is
(a) NH4 (b) Methyl sulphonate
(c) Dimethyle sulphonate (d) DMSO
21 Non coding sequence of gene is called
(a) Recon (b) Exon
(c) Muton (d) Intron
22 Net charge of DNA molecule is
(a) Positive (b) Negative
(c) Neutral (d) none of these
2: Totipotency and morphogenesis, nutritional requirements of in-vitro
cultures
23 Which of the following favors root induction in tissue culture?
(a) Equal auxin to cytokinin ratio (b) High auxin to cytokinin
ratio
(c) Low auxin to cytokinin ratio (d) High cytokinin to auxin
ratio
24 The progeny regenerated from tissue cultured plantlets is denoted as..
(a) R0 (b) R1
(c) R3 (d) R2
25 Agar is isolated from
(a) Bacteria (b) Yeast
(c) Weed (d) Algae
26 The most common carbon source used in plant tissue culture media
is
(a) Fructose (b) Sucrose
(c) Glucose (d) Galactose
27 Shoot proliferation from callus is promoted by
(a) IAA/NAA (b) BAP/BA
(c) GA3 (d) ABA
28 Which of the following is a thermo labile compound
(a) Plant growth regulators (b) Agar
(c) Charcoal (d) Micronutrients
29 Which of the following is not a plant tissue culture nutrient medium
(a) MS (b) Gamborg
(c) B5 (d) S9
30 The unorganized mass of the cells known as
(a) Callus
(b) Embryo
(c) Proliferation (d) Quotient cell
31 The formation of an entire plant from cultured explants
(a) Callus (b) Regeneration
(c) Embryogenesis (d) Organogenesis
32 Excised plant portion used to initiate plant tissue culture
(a) Callus (b) Medium
(c) Explant (d) Cybrid
33 Regeneration of plant from tissue of actively dividing organ like stem
tip
(a) Shoot culture
(b) Meristem culture
(c) Organ culture (d) Cell culture
34 The growth of tissue of living organism in suitable culture medium
(a) Cell culture
(b) Organogenesis
(c) Morphogenesis (d) Tissue culture
35 Commonly used gelling agent is
(a) Gelatine
(b) Vermiculite
(c) Charcole (d) Agar
36 Agarose powder isolated from
(a) Bacteria
(b) Fungus
(c) Algae (d) Yeast
37 The development of a structure from an unorganized state to
differentiated and organized state
(a) Redifferentiation (b) Totipotency
(c) Morphogenesis (d) All of these
38 For filter sterilization pore size should be ……
(a) < 0.45 µ (b) >0.45 µ
(c) >0.45 mm (d) >25mm
39 For explants sterilization …….%Mercuric chloride concentration
generally used
(a) 0.1-1.0% (b) 5-10 %
(c) 0.001-0.01% (d) 20-25%
40 Which of the following chemicals not used for sterilization of explants
(a) Bromine water (b) H2O2
(c) HgCl2 (d) Si
41 ……% ethanol used for sterilization of explants
(a) 10 (b) 20
(c) 50 (d) 70
42 Nutrients required ……………concentration is known as
macronutrients.
(a) >0.5m mol/l (b) >0.005 m mol/l
(c) <0.5m mol/l (d) <0.005m mol/l
43 Nutrients required ……………in concentration is known as
micronutrients.
(a) >0.5m mol/l (b) >0.005 m mol/l
(c) <0.5m mol/l (d) <0.005m mol/l
3: Techniques of in-vitro cultures, micro propagation, anther culture, pollen
culture, ovule culture, embryo culture, test tube fertilization, endosperm
culture and factor affecting in-vitro culture.
44 Polarity of somatic embryo is
(a) Multipolar (b) Monopolar
(c) Bipolar (d) All of these
45 Embryo rescue technique helps to overcome
(a) Post-fertilization barrier (b) Vitrification
(c) Browning problem (d) Pre-fertilization barrier
46 The formation of embryos from callus is known as
(a) Parthenogenetic (b) Indirect embryogenesis
(c) Direct embryogenesis (d) None of these
47 Seed propagation of sterile plant is possible through
(a) Pollen culture (b) Ovary culture
(c) Invitro fertilization (d) Synthetic seed
48 High sucrose level acts as _____ in pollen culture medium
(a) Diffusion (b) Plasmolytic
(c) Osmolytic (d) Osmoregulator
49 Hyper hydration/vitrification problem is associated with
(a) Micropropagation (b) Pollen culture
(c) Embryo culture (d) Ovule culture
50 The development of somatic embryo from other than Meristematic cell
is describe as
(a) Androgenic embryo
(b) Parthenogenetic embryo
(c) Gamatophytic embryo (d) Adventives embryo
51 Induction phase of embryo genesis is promoted by
(a) Auxin
(b) ABA
(c) Cytokinin (d) GA3
52 For embryogenesis which of the following developmental events are in
correct sequence
(a) Heart shapedGlobular
shapetorpedo
shapedcotyledonary stage
(b) Globular shape Heart
shaped Torpedo
shapedCotyledonary
stage
(c) Globular shape Torpedo
shaped Heart shaped
Cotyledonary stage
(d) Heart shaped torpedo
shaped Globular shape
cotyledonary stage
53 Gynogenesis term related to
(a) Fertilized ovule
(b) Unfertilized ovule
(c) Mature ovule (d) Both A and B
54 In which phase the rate of cell division is highest
(a) Lag phage
(b) Linear phase
(c) Exponential phage (d) Stationary phase
55 In which phase the rate of cell expansion is increase
(a) Declaration phase
(b) Linear phase
(c) Exponential phage (d) Stationary phase
56 In which phase the both rate of cell division and expansion are
decline
(a) Declaration phase
(b) Linear phase
(c) Exponential phage (d) Stationary phase
57 In pollen culture medium high sucrose level may act as
(a) Osmoregulatory
(b) Plasmolytic
(c) Osmolytic (d) Diffusion
58 The technique which used to recover hybrid from sexually
incompatible species
(a) In vitro fertilization
(b) In vitro pollination
(c) Embryo culture (d) Unfertilized ovule
59 The ploidy level of primary endosperm cell is
(a) 2n
(b) n
(c) 3n (d) 4n
60 Pre-fertilization barrier in wide hybridization can be overcome by
(a) Embryo culture
(b) Embryo rescue
(c) In vitro pollination (d) Anther culture
61 Virus free plantlets can be produce through
(a) Stem cutting (b) Meristem culture
(c) Organ culture (d) Microspore culture
62 Browning in plant tissue culture media occurs due to
(a) Polyphenols (b) Sugar
(c) Vitamins (d) Charcoal
63 The tendency of callus cells to separate easily is known as
(a) Friability (b) Hybridity
(c) Callusing (d) Granulation
64 Regeneration of plant from tissue of actively dividing organ like stem
tip
(a) Shoot culture
(b) Meristem culture
(c) Organ culture (d) Cell culture
65 The culture used for triploid production is
(a) Pollen (b) Endosperm
(c) Embryo (d) Ovary
66 The production of haploid can be done by
(a) Chromosome elimination (b) Chromosome doubling
(c) Double fertilization (d) All of these
67 In which phase cell attain capacity for embryogenesis and develop up
to globular stage
(a) Developmental
(b) Induction
(c) Maturation (d) Germination
68 The production of haploid embryo or plantlets from pollen grain is
known as
(a) Androgenesis
(b) Androgenetic
(c) Gynogenesis (d) Both A and B
69 Which of the following hormone used for maturation of somatic
embryo
(a) ABA (b) NAA
(c) IBA (d) IAA
70 Generally pH of media adjusted at ……
(a) 3.8 (b) 5.8
(c) 6.8 (d) 7.8
4: Application and achievements of somaclonal variation, types and reasons
71 When somaclonal variation is not heritable, it is called
(a) Epigenetic (b) Genetic
(c) Calliclonal (d) Protoclonal
72 Somaclonal variety of Indian mustard is
(a) Pusa Kiran (b) Pusa Bold
(c) Varuna (d) Pusa Jaikishan
73 The basis of somaclonal variation is
(a) Karyotype change
(b) Transposable element
(c) Gene mutation (d) All of these
74 The variety developed by somaclonal variation in sugarcane for Fiji
disease resistant is
(a) Pindar
(b) Ono
(c) Bio 902 (d) Valvet
75 The variety developed by somaclonal variation in Lytharus sativus for
low neurotoxin (BOAA) is
(a) Pindar
(b) Stephen
(c) Rattan (d) Valvet
76 Mustard Variety Pusa Jaikishan of develop from Varuna through
which techniques
(a) Somatic hybridization
(b) Cybrid
(c) Gamatoclonal variation (d) Somaclonal variation
77 Variability generated in tissue cultured plant is known as
(a) Somatic variation (b) Somaclonal variation
(c) Tissue variation (d) Germinal variation
78 Variation observed among the callus culture is known as …
(a) Calliclonal variation (b) Gametoclonal variation
(c) Protoclonal variation (d) Androclonal variation
79 Variation observed among anther (or) pollen culture is known as
(a) Protoclonal variation (b) Androclonal variation
(c) Androclonal variation (d) Calliclonal variation
80 Variation observed among ovule (or) ovary culture is known as…
(a) Protoclonal variation (b) Gynoclonal variation:
(c) Androclonal variation (d) None of these
81 Variation observed among protoplast culture is known as …
(a) Protoclonal variation (b) Gynoclonal variation:
(c) Androclonal variation (d) None of these
5: Somatic embryogenesis and synthetic seed production technology
82 Fertilized ovule culture will produce
(a) Haploid (b) Diploid
(c) Triploid (d) Pentaploid
83 Synthetic seeds are encapsulated by the chemical
(a) Agar (b) Ca-Alginate
(c) Polypropylene (d) Wax
84 Encapsulation of somatic embryo is required in case of
(a) Somaclonal variation (b) Synthetic seed
(c) Pollen culture (d) All of these
85 The encapsulated somatic embryo is:
(a) Capsulation
(b) Synthetic seed
(c) Somatic seed (d) All of above
86 The concept of synthetic seeds was developed and term used by
(a) Toshiba Murashige (b) Smith
(c) Nathan (d) Maheswari et al
87 Artificial seeds may be produced by following ways…
(a) Desiccated (b) Hydrated systems
(c) None of these (d) a & b both
6: Protoplast isolation, culture, Manipulation and Fusion; Production of
somatic hybrid and cybrids, their application in crop improvement
88 Pomato is an example of
(a) Hybrid (b) Dybrid
(c) Vybrid (d) Cybrid
89 Who used enzyme for protoplast isolation first time
(a) Takebe et al (b) Cocking et al
(c) White (d) Kranz et al
90 The cell devoid of cell wall and nucleus is known as
(a) Cytoplast (b) Protoplast
(c) Cytome (d) Chloroplast
91 In case of protoplast fusion, which of the following stages are in correct
sequence
(a) Agglutination Membrane fusion Rounding off of protoplast
(b) Rounding off of protoplast Membrane fusion Agglutination
(c) Membrane fusionRounding off of protoplast Agglutination
(d) Membrane fusion Agglutination Rounding off of protoplast
92 Application of electric current for protoplast fusion is known as
(a) Microinjection (b) Electrophoresis
(c) Electroporation (d) None of these
93 Spontaneous fusion of protoplasts often produces
(a) Homokaryon (b) Heterokaryon
(c) Vybrid (d) Both (a) and (b)
94 Pectinase enzyme isolated from
(a) Aspergillus niger
(b) Rhizopus spp
(c) Trichoderma viride (d) Trichoderma harsianum
95 Macerozyme isolated from
(a) Aspergillus niger
(b) Rhizopus spp
(c) Trichoderma viride (d) Trichoderma harsianum
96 The somatic hybrid with normal protoplast of one species and nucleus
less protoplast of other species is called
(a) Somatic Hybrids
(b) Cybrids
(c) Vybrids (d) Cytoplast
97 The cellulose enzyme derived from
(a) Fungus
(b) Yeast
(c) Algae (d) Bacteria
98 Most Commonly used fusogen is
(a) Diethyl sulphonate
(b) Sodium nitrate
(c) Poly Ethylne Glycole (d) Isozyme
99 Which of the following are used as osmoticum
(a) Sorbitiol
(b) Manitol
(c) Glucose (d) All of these
100 The osmoticum added in protoplast isolation for
(a) To prevent bursting of
protoplast
(b) Maintain osmotic
pressure
(c) Increase osmosis (d) Reducing osmotic potential
101 The hybrid is produced due to partial hybridization is
(a) Asymmetric hybrid
(b) Symmetric hybrid
(c) Cybrid (d) Vybrid
102 The novel interspecific hybrid-pomato is developed by
(a) Somatic hybridization
(b) Somaclonal variation
(c) Transgenic breeding (d) Conventional breeding
103 The hybrid which developed through fusion of two normal protoplast of
different source
(a) Symmetric hybrid
(b) Cybrid
(c) Asymmetric hybrid (d) Vybrid
7: Genetic engineering, restriction enzyme and vector for gene transfer-gene
cloning.
104 The enzyme which is used for joining DNA fragment is called
(a) S1 Nuclease (b) Nuclease
(c) Ligase (d) DNA polymerase
105 Restriction endonucleases are also known as
(a) Molecular scissor (b) Scalpel
(c) knives (d) Cutter
106 Restriction enzymes protect bacterial DNA by the process of …………..
(a) Methylation (b) Super coiling
(c) Addition of OH group (d) Acetylation
107 The enzyme T4 ligase is generally obtained from
(a) Mycoplasma (b) Fungi
(c) Bacteriophage (d) Bacteria
108 The restriction endonucleases were discovered by
(a) Sharp and Robert (b) Hedertberg and Meselon
(c) Smith and Nathans (d) Temin and Baltimore
109 BamHI is belong to which category of Restriction enzyme
(a) Type-III (b) Type-I
(c) Type-II (d) Type-IV
110 Restriction endonucleases having identical recognition sequence but
different cleavage sites are called
(a) Schizomers (b) Isoschizomers
(c) Neomers (d) Neoschizomers
111 Which of the following is DNA modifying enzyme ?
(a) S1 Nuclease (b) Cellulase
(c) Protease (d) RNAse
112 DNA ligase joins two DNA molecules by formation of which bond
(a) Peptide (b) Disulphide
(c) Phosphodiester (d) Hydrogen bond
113 Recognition sequence of HindIII is
(a) 5' GAATTC 3'
3' CTTAAG5'
(b) 5' AGCT 3'
3' TCGA 5'
(c) 5' AAGCTT 3'
3' TTCGAA 5'
(d) 5' TCGA 3'
3' AGCT 5'
114 Widely used restriction endonuclease for gene cloning is?
(a) Type-III (b) Type-I
(c) Type-II (d) Type-IV
115 Hind III is belong to which category of Restriction enzyme
(a) Type-III (b) Type-I
(c) Type-II (d) Type-IV
116 EcoR1 is a ………… restriction enzyme
(a) Type-III (b) Type-I
(c) Type-II (d) Type-IV
117 The enzyme which degrades nucleic acid is?
(a) Ligase (b) Nucleases
(c) Kinase (d) S1 Nuclease
118 The enzyme used to synthesis of cDNA by using mRNA as template
(a) Transcriptase (b) Reverse transcriptase
(c) DNA polymerase (d) RNA polymerase
119 The complementary DNA obtained from RNA molecules is known as
(a) cDNA (b) tDNA
(c) rDNA (d) RNA
120 The DNA is visualized after electrophoresis under U.V radiation due to
(a) Bromophenol blue (b) Tris HCL
(c) Chloromphenicol (d) Ethidium bromide
121 The enzyme reverse transcriptase was discovered by
(a) Temin and Baltimore (b) Maxam and Gilbert
(c) Smith and Nathan (d) Sharp and Robert
122 The process of removal of non-coding sequence from mRNA is
(a) RNA editing (b) Capping
(c) Tailing (d) Splicing
123 Which of the following dye is used as tracking dye during
electrophoresis?
(a) Ethidium bromide (b) Tris HCL
(c) Chloromphenicol (d) Bromophenol blue
124 The site recognized by restriction endonuclease are called
(a) Restriction sequence
(b) Restriction sites
(c) Recognition sites (d) All of these
125 The enzymes that cleaves or cut nucleic acid at internal sites.
(a) Endonuclease (b) Exonuclease
(c) S1 nuclease (d) Exonuclease III
126 Restriction endonuclease with identical recognition and cleavage sites
are called
(a) Schizomers (b) Isoschizomers
(c) Neomers (d) Neoschizomers
127 The molecule or segment produced by joining together two or more DNA
segments from different organism is?
(a) rDNA (b) rRNA
(c) cDNA (d) DNA
128 Enzyme used for removal of RNA from DNA is known as …
(a) Ligase (b) Methylase
(c) Pectinase (d) RNAse
129 Post transcriptional modification is known as…..
(a) RNA editing (b) Capping
(c) Tailing (d) Splicing
130 Coding sequence of gene is
(a) Intron
(b) Exon
(c) Muton (d) Recon
131 The enzyme reverse transcriptase isolated from
(a) Mycoplasma (b) Bacteria
(c) Retroviruses (d) All of these
132 1to 15 Kb size of DNA fragment can be accommodated in…..
(a) Phagemids (b) Plasmid
(c) BAC (d) YAC
133 The organism which is used for cloning foreign DNA in bacteria is called
(a) Vector (b) Small genome
(c) Chromosome (d) Viruses
134 The vector used for production of the protein specified by the DNA insert
is ?
(a) Shuttle vectors (b) Cloning vectors
(c) Expressing vectors (d) None of these
135 The number of plasmid present per bacterial cells is called
(a) Complementary number (b) Code number
(c) Copy number (d) Cose site number
136 Vector having common characteristics of both Phage and plasmid is…..
(a) pUC 18 (b) YAC
(c) Phagemid (d) pBR322
137 The cloning capacity of BAC vector is………..
(a) 150-300kb (b) 400kb
(c) 400-3000 kb (d) 400-1000 kb
138 Which of the following is not a type of vector?
(a) Plasmid (b) Phagemid
(c) Cosmid (d) Transmid
139 The most of the vectors used for transformation of DNA fragment in
plant is based on
(a) Bacteriophages (b) YAC
(c) BAC (d) Ti plasmid
140 The vector which have ability to replicate in cells of two different species
or host
(a) Cosmid (b) Phagemids
(c) Expressing vector (d) Shuttle vector
141 Complementary molecules of DNA join to each other by …….. ?
(a) Peptide (b) Disulphate
(c) Phosphodiaster (d) Hydrogen bond
142 The largest size of DNA fragment can be accommodate in
(a) Phagemids (b) Plasmid
(c) Cosmids (d) YAC
8: Direct and indirect method of gene transfer, transgenic plants and their
application, and blotting techniques
143 Agrobacterium is
(a) Plant bacterium (b) Gram negative soil
bacterium
(c) Gram positive soil bacterium (d) Both A and B
144 Which of the following plasmid of Agrobacterium rhizogenes?
(a) Ti (b) Ri
(c) pBR322 (d) pUC
145 Which of the following is not a direct transformation technique?
(a) Agrobacterium mediated (b) Microinjection
(c) Electroporation (d) Microprojectile
146 PEG is used in which of the following gene transfer methods?
(a) Microinjection (b) Biolistic
(c) Electroporation (d) Chemical mediated
147 Which of the following gas is used in microprojectile method?
(a) Carbon dioxide (b) Helium
(c) Nitrogen (d) Oxygen
148 Which part of the Agrobacterium is transferred in the host plant
cells
(a) Vir region (b) T-DNA
(c) Resistance gene region (d) Opine gene region
149 Gene gun method is also known as
(a) Electroporation (b) Particle bombardment
(c) Agrobacterium mediated (d) Microinjection
150 Agrobacterium tumefacians infected only dicot plant due to which
chemical compounds
(a) Acetosyringone (b) Acetyle
(c) Acetogene (d) Syringone
151 In fiber mediated transformation DNA is delivered into cell
cytoplasm and nuclease by
(a) Fiber (b) Injection
(c) PEG (d) Silicon carbide fibers
152 In particle bombardment method for delivering a foreign DNA into
target plant cell by using
(a) Helium gas (b) Electric voltage
(c) PEG (d) Lipid vesicles
153 DNA directly injected into the plant protoplast or cells using fine
tipped glass needles
(a) Electroporation (b) Gene gun
(c) Micro injection (d) Electrophoresis
154 Particle widely used in gene gun methods is made up by
(a) Gold (b) Silver
(c) Mercury (d) Iron
155 Application of an electric current to cell for transforming DNA is?
(a) Electroporation (b) Gene gun
(c) Micro injection (d) Electrophoresis
156 Introduction of DNA into cells via small lipids artificial vesicles is
called
(a) Liposomes (b) Lypofection
(c) lysosomes (d) Both A and B
157 The introduction or transfer of DNA into the cells of an organism
by a method other than sexual crossing
(a) Translation (b) Transformation
(c) Transduction (d) Both B and C
158 Transgenic for male sterility-fertility mainly concerned with which
genes?
(a) Cry-II (b) Cry-I
(c) Barnase and Barstar (d) Cry-IAc
159 The Cry-I protein are insecticidal to
(a) Lepidopteran insect (b) Dipteran insect
(c) Coleopteran insect (d) Both A and B
160 Flavr-Savr is a popular example of which transgenic crop?
(a) Potato (b) Tomato
(c) Pomato (d) Brinjal
161 Roundup ready is popular example of which transgenic crop?
(a) Cotton (b) Wheat
(c) Soybean (d) Both a and c
162 Bollgard variety of cotton resistant against which insect- pest?
(a) Cotton bollworm (b) Tobacco bud worm
(c) Pink boll worm (d) All of these
163 Round-up ready crop plant tolerant to which herbicide?
(a) Glyphosate (b) Pendemethalin
(c) 2-4-D (d) Paraquat diquat
164 Transgenic for male sterility-fertility mainly concerned with which
genes?
(a) Barnase (b) Barstar
(c) Barnase and Barstar (d) None of these
165 Golden rice accomplishes the requirement of which of the
following?
(a) Vitamin B (b) Vitamin A
(c) Vitamin A and D (d) Vitamin 12
166 The second generation of transgenic plant is denoted by
(a) T0 (b) T2
(c) T1 (d) T3
167 Flavr-Savr tomato having characteristics to
(a) Good test (b) Deep red colour
(c) Fast mature (d) Delay ripening
168 The barnase and barstar gene isolated from
(a) Bacillus amylolecufacians (b) B. thurengiensis
(c) Agrobacterium rhizogine (d) Thermus equatics
169 Bt gene isolated by
(a) Barton et al (b) Fodor
(c) Williams (d) Powell
170 Bt gene isolated from
(a) Plant bacterium (b) Soil bacterium
(c) Organism (d) Air spores
171 The plant developed with a foreign gene is
(a) Transformed plant
(b) Transplanted plant
(c) Transgenic plant (d) GMO
172 The Cry-I protein are insecticidal to
(a) Lepidopteran insect (b) Dipteran insect
(c) Coleopteran insect (d) Both A and B
173 Golden rice is a good source of
(a) Vit-A (b) Amino acid
(c) Vit-B (d) Starch
174 Which of the following cry protein can be used for protection
against Lepidoptera insects ?
(a) CryI (b) Cry II
(c) Cry III (d) Both A and B
175 Which of the following promoter is used for seed specific
expression?
(a) 35S (b) TA29
(c) Adh1 (d) Vicilin
176 Which of the promoter specific for anaerobic expression?
(a) Adh1 (b) TA29
(c) Vicilin (d) Crt1
177 The first transgenic plant was produced in which of the following
crop
(a) Tomato (b) Cotton
(c) Tobacco (d) Maize
178 The first transgenic was produced which of the following character
(a) Ampicillin resistance (b) Neomycin resistance
(c) Kanamycine resistance (d) Hygromycine resistance
179 In which year first transgenic plant was developed
(a) 1973 (b) 1983
(c) 1993 (d) 2003
180 In which of the following crop transgenic variety Flavr Savr was fist
reach to market
(a) Tomato (b) Cotton
(c) Tobacco (d) Maize
181 Which of the following blotting method is used for protein?
(a) Southern (b) Northern
(c) Western (d) Eastern
182 The technique used to separate DNA fragments is
(a) Southern blotting (b) Gel electrophoresis
(c) SSR (d) PCR
183 Transfer of nucleic acid fragment from gel to nitrocellulose or nylon
membrane is known as
(a) Labelling (b) Probing
(c) Blotting (d) Hybridization
184 A procedure in which RNA fragment are transferred from an
agarose gel to nitrocellulose membranes or DBM membrane is
known as …
(a) Northern blotting (b) Southern blotting
(c) Western blotting (d) Both A and B
9: DNA finger printing, DNA based markers RFLP, AFLP, RAPD, SSR, and
DNA probe
185 Invitro amplification of DNA is done by
(a) Polymerase Chain Reaction (b) Polymerase Chain
Reduction
(c) Gel documentation system (d) Gel electrophoresis
186 Annealing step of PCR involves
(a) Separation of two strands (b) Rearrangement of DNA
(c) Joining of primer to
complimentary strand
(d) Super coiling
187 Extension step of PCR occurs at which temperature?
(a) 90 oC (b) 72 oC
(c) 60 oC (d) 94 oC
188 Which of the following is not used for DNA finger printing
(a) RAPD (b) RFLP
(c) SSR (d) Blotting
189 Short single stranded oligonucleotide that is used for DNA amplification
in PCR is
(a) cDNA (b) Primer
(c) QTL (d) Probe
190 The technique used to separate DNA fragments is
(a) Southern blotting (b) Gel electrophoresis
(c) SSR (d) PCR
191 Which of the following is not a PCR based marker?
(a) RAPD (b) RFLP
(c) SSR (d) ISSR
192 The fragment of DNA is separated by
(a) Chromatography (b) Electrophoresis
(c) Electroporation (d) PCR
193 A small nucleotide sequence of DNA or RNA used to detect presence of
complimentary sequence from nucleic acid sample
(a) Promoter (b) Regulatory sequence
(c) Marker (d) Probes
194 The PCR techniques was developed by
(a) Kary Mullis (b) Smith and Nathan
(c) Craig ventor (d) Maxam and Gilbert
195 The enzyme used in PCR is
(a) TaqDNA polymerase (b) DNA polymerase I
(c) DNA polymerase II (d) Reverse transcriptase
196 TaqDNA polymerase isolated from
(a) B. amyloliquefaciens (b) Thermus aquatics
(c) B. tinctora (d) B. thermis
197 Inheritance patterns of RAPD are?
(a) Dominant (b) Co dominant
(c) Non-expressing (d) Neutral
198 The techniques of determining the order of bases of DNA molecules?
(a) DNA hybridization (b) DNA sequencing
(c) DNA ligation (d) DNA blotting
199 Which of the following is co-dominant DNA markers?
(a) RFLP (b) RAPD
(c) SSR (d) Both a and c
200 RFLP was the first molecular marker that was developed in …….
(a) 1990 (b) 1880
(c) 1980 (d) 1890
201 Which of the following is hybridization based marker?
(a) RFLP (b) AFLP
(c) RAPD (d) SSR
202 Sequence information is not required for
(a) SSR (b) RAPD
(c) SNP (d) ESTs
203 Single decamer primer used for which of the molecular marker
(a) RAPD (b) SSRs
(c) RFLP (d) ISSR
204 RAPD marker annealed to the template DNA typically at ………
temperature.
(a) 37°C (b) 55°C
(c) 65°C (d) 72°C
205 AFLP is a …..type of marker.
(a) Dominant (b) Co-Dominant
(c) Partially co-dominant (d) None of these
206 Polymorphism in SSR locus typically due to….
(a) Variation in repeat motif (b) Single nucleotide
polymorphism
(c) Large insertion or deletion (d) None of these
207 For separation of RAPD marker typically……..% gel is required
(a) 1% (b) 1.5%
(c) 3% (d) 5%
208 RAPD marker was developed by
(a) Vos et al (b) Fodore
(c) Williams et al (d) None of these
209 Which of the following marker is low reproducible
(a) SSR (b) RAPD
(c) SNP (d) AFLP
210 Variation in SSR motif generated due to ….
(a) Replication slippage (b) Unequal recombination
(c) Transcription (d) Both a & b
211 Which of the following marker known as multi allelic marker
(a) SSRs (b) RAPD
(c) AFLP (d) ISSR
212 Microsatellites are repeats of
(a) 1-6 bp (b) 5-10 bp
(c) 10-20 bp (d) 20-25 bp
10: Mapping QTLs –Future prospects, MAS, and its application in crop
improvement
213 In 1975 the term QTL was coined by……
(a) Smith (b) Gelderman
(c) Nathan (d) Khurana
114 Region or locus on the chromosome associated with the expression of a
quantitative trait is called as
(a) BIM (b) MCS
(c) Intron (d) QTLs
215 Which of the following varieties is the first in India that were developed
by Marker Assisted selection?
(a) Improved Pusa Basmati 1
(Pusa 1460)
(b) Sabarmati
(c) Pusa Basmati (d) IR64
216 Which of the following character was transferred in Improved Pusa
Basmati 1 through MAS?
(a) Heat tolerance (b) Bacterial Leaf Blight
Resistance
(c) Cold tolerance (d) Insect Resistance
217 Gene……………incorporated in Pusa Improved Basmati
(a) Xa21 (b) Xa 13
(c) Xa 1 (d) a and b both
218 Which of the following is required for QTL mapping
(a) Mapping Population (b) Polymorphic molecular
marker
(c) Phenotypic data (d) All of these
219 Which of the mapping population is immortal or permanent
(a) DH (b) F2
(c) RILs (d) a & c both
220 In which of the mapping population heterosis effect of locus can be
observed
(a) F2 (b) NILs
(c) RILs (d) DH
221 Which of the following is utilized as mapping population?
(a) Backcross Line (b) Recombinant Inbred Line
(c) Double Haploid (d) All of these
222 Which of the following method used for QTLs identification?
(a) Single marker approach (b) Composite interval
mapping
(c) Simple interval mapping (d) All of these
223 Software package used for QTL detection
(a) QTL cartographer (b) Bioedit
(c) Nextgen (d) Mega 4.2
224 Distance of markers in linkage map is defined by unit of
(a) cM (b) kM
(c) gM (d) pM
225 Software package used for construction of linkage map
(a) MAPMAKER (b) Bioedit
(c) GMAP (d) Mega 4.2
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