Genomics in plant breeding (Question Paper )

 

 

Answer the following:

1. Describe the steps involved in DNA marker-based linkage map construction. (5.00 marks)
2. Mention the principle of single marker analysis for detecting QTL with a numerical example. (4.00 marks)
3. Define mapping function and its use and mention types of mapping functions and the differences between them. (6.00 marks)
4. Describe the criteria to arrive at the most probable order of markers assigned to a linkage group with examples. (6.00 marks)
5. If 15 markers are assigned to a linkage group, estimate all orders possible. (1.00 mark)
6. Mention the requirements of mapping QTL controlling traits of economic importance. (3.00 marks)
7. If the frequency of recombination between two genes is 0.1, estimate the map distance between them based on Haldane and Kosambi mapping functions. (2.00 marks)
8. Two QTLs were detected on rice chromosomes 7 and 8 with LOD scores of 5 and 3. Interpret the statement. (2.00 marks)
9. Mention the most appropriate marker systems for genotyping F₂, RIL, Backcross, NIL, di-haploid populations, and immortal F₂ populations. (6.00 marks)