Time: 90 minutes
Max Marks: 90
SUBJECT: GENETICS AND PLANT BREEDING
1. Transcriptomes
a. Consist of RNA
b. Consist of proteins
c. Translated proteins
d. None of the three
2. Relationship between chromosomes related by dissent in wheat are called
a. Homologous chromosomes
b. Homocologus chromosomes
c. Heterologous chromosomes
d. Orthologous chromosomes
3. The following do not have RNA
a. Cytoplasm
b. Ribosome
c. Chromosome
d. Nucleolus
4. Absence of one whole chromosome from a diploid is called
a. Trisomic
b. Tetrasomic
c. Monosomic
d. Isochoromosome
5. In auto-triploids the number of linkage groups is
a. 2n
b. n
c. 3n
d. 4n
6. The step in the flow of genetic information where in DNA is copied into mRNA is called
a. Translation
b. Transformation
c. Replication
d. Transcription
7. The terminator sequence in genes prevent seed germination
a. In second season
b. Immediate generation
c. After three generation
d. Kills the seed after harvest
8. Addition or deletion of one or two bases to a DNA strand causes
a. Frame shift mutation
b. Chromosome mutations
c. Insertions
d. Major mutations by additions
9. Independently assorted characters
a. Haye-linked genes
b. Segregate independently
c. Partially linked
d. Linkage is 20 percent
10. The operon hypothesis was proposed by \( \neg Jacob \, ? \, Moran \, \lambda \)
a. Watson and Crick \( \rightarrow double \, helix \, medi. \)
b. Beadle and Tatum
c. None
d. Joseph and Morgan
11. In mitosis, DNA synthesis takes place during
a. G1 stage
b. S Stage
c. G2 Stage
d. All of the above
12. The process of DNA synthesis from RNA is called
a. Reverse translation
b. Reverse transcription
c. Transcription
d. Mutation
13. How many different genotypes can exists in a population with dominance hierarchy
\g^a > g^b > g^c > g^d
a. 6
b. 8
c. 16
d. 20
14. Inversion leads to alternation in
a. Composition of genes
b. Sequence of gene
c. Number of genes
d. Addition of bases
15. Translocation homozygotes lead to alternation in
a. Gene number
b. Sequence of genes
c. Linkage map
d. Linkage groups
16. A cross made between the F\(_1\)'s of two inbred pairs of different origin is called
a. Tests cross
b. Double cross
c. Reciprocal cross
d. Dominant cross
17. Highly cross pollinated species among the following is
a. Maize
b. Rice
c. Chilli
d. Cotton
18. Transposons contain
a. RNA sequences
b. DNA sequences
c. Only found in eukaryotes
d. Contain no genes
19. Scientific name of indica rice is
a. Oryza sativa
b. Oryza indicum
c. Oryza japonica
d. Oryza indica
20. An amino acid that cannot participate in alpha helical formation is
a. Proline
b. Histidine
c. Phenylalanine
d. Threonine
21. Most genes are transcribed into
a. t-RNAs
b. mRNA
c. Ribosomal RNAs
d. Repeat elements
22. Which process involves the deliberate alteration of an organism’s genetic material?
a. Bioremediation
b. Biotechnology
c. Decomposition
d. Recombinant DNA
23. Genetic markers are
a. Must be repeat DNA sequences
b. Are altered by environment
c. Are only used in genetic maps
d. None
24. DNA polymerases
a. Join DNA fragments
b. Replicate RNA
e. Replicate DNA
d. Synthesize DNA in 5’->3’ direction
25. The basic principle used in PCR was putforth by
a. Meselson and Stahl
b. Kary Mullis
c. Watson and Crick
d. Hudson and Stahl
26. Plant DNA finger printing can be most effectively carried using
a. ISSR
b. AFLP
c. SNP
d. SSR
27. Micro propagation is associated with
a. Tissue culture
b. Vermiculture
c. Silviculture
d. Sericulture
28. The size of mutan in Benzer fine structure of gene
a. 1bp
b. 10bp
c. 100bp
d. 1000bp
29. Main architects of Golden rice
a. Ingo potrykus and Peter Beyer
b. M. S. Swaminathan and G. S. Kush
c. G. S. Kush and Robert Ziegler
d. Robert Ziegler and M. S. Swaminathan
30. Which enzyme of the following was isolated from Bacteria?
a. Taq polymerase
b. dNTP Polymerase
c. Exon polymerase
d. Taq Isomerase
31. Agarose is a
a. Polysaccharide
b. Polydecoaharide
c. Polyacylamide
d. Polyamide
32. Standard gel electrophoresis of DNA separates DNA fragments based on
a. Charge differences.
b. Sequence differences at one nucleotide.
c. Size differences.
d. Type of DNA
33. The segment of gene that codes for protein
a. Intron
b. Exon
c. ISSR
d. Nion
34. The principle of Marker Assisted Selection is
a. Marker ratio
b. Linked genes
c. Marker Free Selection
d. Crossing over region
35. RNA virus with the ability to convert its sequence into DNA by reverse transcription
a. Tobacco mosaic Virus
b. Retrovirus
c. Ring spot virus
d. Provirus
36. Protein that oversees the correct binding of other proteins
a. Molecular neutrons
b. Molecular cutters
c. Molecular chaperone
d. Molecular scissors
37. An enzyme that uses single stranded RNA as a template for making double stranded DNA
a. Reverse Transcriptase
b. Reverse Transase
c. Reverse Phosphatase
d. Reverse Criptase
38. DNA sequence found within genes that are involved in the regulation of patterns of development (morphogenesis) in animals, fungi and plants are called
- Homeobox
- Herterobox
c. Homobox
d. Holobox
39. To distinguish a double cross over product we need at least
a. Two genes on a linkage group
b. Three genes on a linkage group
c. Three genes on two linkage groups
d. Four gene on a linkage group
40. DNA methylation involves the
a. Deletion of methyl group
b. Addition of methyl group
c. Neither deletion nor addition
d. Addition of ethyl group
41. Which is the correct order of the taxonomic categories, going from most specific to most general?
a. domain, kingdom, phylum, class, order, family, genus, species
b. division; domain, kingdom, class, family, genus, species
c. species, genus, family, order, class, phylum, kingdom, domain
d. species, family, class, order, phylum, kingdom
42. Enzyme that changes the topology of DNA by nicking and resealing one strand of DNA
a. Topoisamerae I
b. Topoisamerae II
c. Topoisamerae III
d. Topoisamerae IV
43. Movement of a transposon to a new site in the genome
a. Transposition
b. Transduction
c. Transcription
d. Translation
44. Relationship between chromosomal regions of different species where homologous genes occur in the same order
a. Monectomy
b. Syntony
c. Parasynteny
d. Synonym
45. Enzyme that functions in tRNA splicing
a. RNA ligase
b. RNA galactase
c. RNA lactase
d. RNA lucase
46. Enzyme that is responsible for a site-specific recombination
a. Integrase
b. Intergrase
c. Innase
d. Invertase
47. Which episome of the bacterium *Agrobacterium tumefaciens* that carries the genes responsible for the induction of crown gall disease in infected plants
a. Golgi complex
b. T1 Plasmid
c. R1 Plasmid
d. Pi Plasmid
48. A coding system in which each word may be coded by a variety of symbols or groups of letters is said to be
a. Archaic
b. Redundant
c. Degenerate
d. Polysyllabic
49. Two poly nucleotide chains of DNA are linked by
a. Hydrogen bond
b. Phosphodiester bond
c. Peptide bond
d. Glycoside bond
50. An amino-acyl synthetase is responsible for
a. Formation of peptide bond
b. Attaching an amino group to an organic group
c. Movement of t-RNA molecule from A to P site on a ribosome
d. Joining an amino acid to a t-RNA
51. The growing polypeptide chain is first attached to a tRNA molecule in which site of the ribosome
a. A site
b. B site
c. P site
d. Active site
52. With higher annealing temperature PCR amplification becomes
a. More specific
b. Less specific
c. No effect
d. Average specific
53. Central dogma of molecular genetics was proposed by
a. Watson
b. Crick
c. Morgan
d. Pasteur
54. DNA in Bacteria is
a. Circular Ds DNA
b. Circular Ss DNA
c. Linear Ds DNA
d. Linear Ss DNA
55. Genes for rRNA synthesis present in
a. Nucleolus
b. Telomere
c. Centromere
d. Pelicle
56. Non epistatic interaction was given by
a. Morgan
b. Sturtevent
c. Bateson and Punnet
d. Sutton and Boveri
57. Linkage can be detected by
a. Only test corss
b. Only F\(_2\) data
- a. Both test cross and \(F_2\) data
- d. Reciprocal cross
58. A PCR reaction is initiated with 20,000 strands and 0.25ng of DNA, after 6 cycles the number of DNA strands will be ______
a. 3,20,000
b. 6,40,000
c. 64,000
d. 64,00,000
59. Transduction was given by
a. Beadle and Tatum
b. Zinder and Lederberg
c. Lederberg and Tatum
d. Avery-Macleod and McCart
60. Back cross breeding was proposed by
a. Stahl
b. Meselson and Harlan
c. McCury and Johnson
d. Harlan and Pope
61. Bacterial colonies in a congenial nutrient media normally (up to a certain stage of course) multiply in
a. Multiply in geometric ratio
b. Multiply in arithmetic ratio
c. Exponential ratio
d. Multiple ratio
62. Computer program used for analyzing QTL is
a. Cartographer
b. NTSYS
c. STATSTICA
d. Spar
63. If incompatibility is controlled by genotype of the plant, then it is
a. Sporophytic
b. Gametophytic system
c. Aponictic system
d. Gametophytic
64. Pseudodominance is exhibited by
a. Translocation Heterozygote
b. Deficiency Heterozygote
c. Deficiency homozygote
d. Inversion homozygote
65. Y- linked trait exhibiting
a. Criss-cross type of inheritance
b. Uniparental inheritance
c. Qualitative inheritance
d. Quantitative inheritance
66. Supplementary gene interaction is also called
a. Dominant epistasis
b. Duplicate Dominant
c. Duplicate recessive lowplimenty
d. Recessive epistasis
67. Operon model of gene regulation in E. coli was discovered by
a. Jacob and Monod (1961)
b. Temin and Baltimore (1971)
c. Britten and Davidson (1969)
d. Watson and Crick (1953)
e. Dain double helix needle
68. In a primary trisomic the extra chromosome is
a. Isochromosome
b. Translocated chromosome
c. B Chromosome *
d. Per centric chromosome
69. Permanent mapping populations are represented by
a. Back cross generation
b. Recombinant inbred lines
c. F2 generation
d. F1 plants
70. Cis and Trans position are represented by the following order (respectively)
a. AB/ab, Ab/BB
b. AA/BB, BB/AA
c. AA/BB, AA/BB
d. AA/AA, BB/BB
71. Exonucleases chew-up the DNA from
a. inside to outside
b. outside to inside
c. Top to bottom
d. Bottom o top
72. Genes can be transferred from one organism to other by
a. Mutation
b. Pre-breeding
c. Negative selection
d. Agro bacterium tumefaciens
73. Registration of Indian varieties must be done with
a. IBPGR
b. NBPGR
c. IARI
d. FAO
74. The process of synthesis of mRNA from a DNA template is known as
a. Translation
b. Transcription
c. Transduction
d. Transformation
75. Nucleic acid was discovered and named by
a. Flemming and Altman
b. Altman and Flemming
c. Wolff and Musicher
d. Meischer and Altman
76. Which of the following single strand would be part of a palindrome in double stranded DNA?
a. GAATTC
b. ATGATG
c. CTAATC
d. CCCTTT
77. Which of the following is an enzyme used to form a phosphodiester bond in a nick between 3' end of one DNA chain and a 5' end of another?
a. DNA polymerase
b. SI nuclease
c. DNA ligase
d. DNA Helicase
78. Bacterial cells are rendered more permeable to uptake of plasmids by treatment with
a. Heat
b. Calcium chloride
c. Alkali
d. Ultrasound
79. The melting temperature of a DNA molecule is determined by using
a. Electrophoresis
b. Change in electrical conductivity
c. Change in optical density
d. Density gradient ultracentrifugation
80. Using distant related wild relatives in crop improvement is known as
a. Pre-breeding
b. Anticipatory breeding
c. Future breeding
d. Contemporary breeding
81. Sanger method of DNA sequencing is also called
a. Enzymatic method
b. Chemical cleavage
c. Automated sequencing
d. Pyrosequencing
82. The classical 1957 experiment of Meseison and Stahl was concerned with
a. Mode of DNA replication
b. Polymerase chain reaction
c. In-vitro production of recombinant DNA molecules
d. Transduction via lambda phage
83. Which of the following is a co-dominant marker?
a. AFLP
b. RAPD
c. RFLP
d. DAF
84. There are three kinds of RNA polymerases (I, II, III) in eukaryotic cells, each specific for one class of RNA molecule (mRNA, tRNA, rRNA) which of the following is a correct match
a. I=rRNA, II-tRNA
b. I=mRNA, III=rRNA
c. I=rRNA, III=rRNA
d. I=rRNA, II=mRNA
85. Which of the following differentiates eukaryotic DNA replication from prokaryotic replication?
a. Multiple origin of replication
b. Bidirectional replication fork
c. No use of an RNA primer
d. Use of only one DNA polymerase
86. Sometimes RAPD marker is also used to detect true \( F_i \)'s of a cross.
a. In a double cross
b. In a distant cross
c. If the female parent generates a band
d. If the male parent generates a band
87. Existence of more than two alleles at a locus is referred to as
a. Pseudo alleles
b. Multiple alleles
c. Isod alleles
d. Triple alleles
88. In Marker – Assisted backcross breeding the number of generations are reduced mainly by selection of
a. Target genes
b. Undesired linked alleles
c. Background alleles
d. Overlapping alleles
89. DNA is the transforming principle was proposed by
a. Watson and Crick (1953)
b. Griffith (1928)
c. Avery, MacLeod and McCarty (1944)
d. Beadle and Tatum (1941)
90. Approximate size of rice genome is –
a. 600 Mb
b. 250 Mb
c. 434 Mb
d. 150 M
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