BIOTECHNOLOGY FOR CROP IMPROVEMENT - GPB – 509 (Question Paper )

  

  

a. Definition (1x1.00 = 1.00)

1. What are haploids? How are they generated?
2. What is toipotency? How is it used in crop improvement?
3. What are the advantages of doubled haploids?
4. Explain SSR markers.
5. What is the use of loading dye in gel electrophoresis?

b. Reasoning and short answers questions (1x2.00=2.00)

1. What is a Vector? Name two vectors which can be used for cloning genes.
2. What is Multi-Cloning Site (MCS)?
3. Define Plasmid.
4. Name two large size vectors.
5. Name two restriction enzymes.

c. Brief Notes / Short notes (1x3.00=3.00)

1. What is transgenic plant? Under what circumstance it is essential to go in for developing transgenics?
2. How can transgenic be stabilized and made homozygous?
3. Define RIL’s. How they are generated.
4. Define the genetics of markers with examples.
5. What is co-segregation? Explain.

d. Long answers questions (1x5.00=5.00)

1. What is Marker Assisted Selection? Write its principles.
2. Write five advantages of Marker Assisted Selection.
3. How are markers useful in backcross breeding?
4. Write five factors affecting agarose gel electrophoresis.
5. What is genotyping and phenotyping in gene mapping?