GPB 603 (2+1) Genomics in Crop Improvement

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Division of Genetics
ICAR-Indian Agricultural Research Institute, New Delhi

Sub: GP603                  Total marks: 30
                         Time: 60 min
Paper 3

1. What is an immortalized F2 population? How it is developed and what advantages it offers in mapping QTLs compared to F2, RILs and DH? (5 marks)

2. Explain why 10 plants are bulked in BSA (kindly provide the reason, not just providing the equation)? (5 marks)

3. Differentiate the following: (10 marks)
    a. SSR and ISSR
    b. RAPD and AFLP
    c. F2 and F2:3
    d. SIM and CIM
    e. RIL and DH

4. A QTL mapping experiment was conducted using single marker analysis to map the QTL governing the trait T1 using the marker M1. Help the experimenter using the following table of ANOVA in generating conclusions (10 marks)
    a. whether M1 is linked to the trait T1?
    b. If yes, what is the percent phenotypic variance explained by the QTL?

Source of Variation	df	SS	MSS	F-test
Between groups	2	58.65
Within group	8	15.25

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