MBB502 Mid-Term
Time: 11:15am to 12:15pm
Q1. Explain the following with diagram:
a) Role of Sigma subunit in recognition of promoter
b) Rho-dependent termination of transcription
Q2. Write short answers for the following
a) Mendel described the genetics of pea crosses, although he did not know what genetic material was. What he described it as, which gave his pea plants their physical characteristics.
b) Nettie Stevens, Walter Sutton and Edmund Wilson, working with the giant chromosomes of Brachystola (grasshoppers) formulated which theory.
c) Quantitative analysis showed what composition of chromosomes….
d) Frederick Griffith (1928) working with the Dipplococcus pneumoniae found what forms of the bacterium………………..
e) Hershey and Chase performed their famous blender experiment using radioactivity for labeling. Which radioisotopes were used to label what?
Q3. With the help of a diagram, explain the Avery, MacLeod, McCarty Experiment to identify the transforming principle.
(5 marks)
Q4. Write short notes on
a) Characteristic features of RNA polymerase II promoters
b) Enhancers and Mediator complex
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