UNIVERSITY OF AGRICULTURAL SCIENCES
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Molecular Breeding GPB 521 (2+0)
Final Examination 2017-18
1. Fill the blanks. (6.0 Marks)
a) BLAST is ___________
b) Genomic representations consists of ___________
c) SWISSPROT is ___________
d) LAMP is ___________
e) The primers used in LAMP are ___________
f) MAGIC is ___________
g) The bottom-up approach tells about ___________
h) Commercially available sequencing technologies are ___________
i) The levels of gene annotation are ___________
j) CDPs is ___________
k) IRAP is ___________
2. Write the functions of orphan genes. (3.0 Marks)
3. Mention the applications of DArT. (2.0 Marks)
4. Why gene identification is difficult in eukaryotes than prokaryotes. Give reasons. (3.0 Marks)
5. Discuss importance and limitations of genome annotation. (3.0 Marks)
6. Write salient features of LAMP. (2.0 Marks)
7. Discuss the steps in MAGIC population generation. (3.0 Marks)
8. Write the procedure to carry out allele mining. (3.0 Marks)
9. How allele mining can be used for crop improvement. (2.0 Marks)
10. Write applications of nanobiotechnology. (2.0 Marks)
11. Compare 1st generation and 2nd generation sequencing technologies. (3.0 Marks)
12. Write note on VIGS. (2.0 Marks)
13. Write applications of: (2.0 Marks)
a) NGS
b) SAGE
14. Write note on: (4.0 Marks)
a) EST-SSR and
b) cDNA AFLP.
c) SCOT and
d) RGAP.

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