1. What is the purpose of QTL mapping and explain in details how do you do QTL mapping?
2. What are molecular markers, its types and applications?
3. What do you mean by molecular marker and explain how do you make molecular markers? List software’s for marker development?
4. What is marker, types of markers and write detail advantages and disadvantages?
5. What is mapping population; give in brief different mapping populations with their advantages and disadvantages? What are the key points to be considering during choice of mapping population?
6.
Explain the procedure of MABC with taking your
own example for improvement of mega varieties for a trait of interest?
7.
MCQ (Write only answer in your answer booklet)
1.
Restriction fragment length polymorphisms
(RFLPs)
a)
Are used to determine the position of
restriction sites in a genome
b)
Are used in physical mapping
c)
Are used in genetic mapping
d)
Usually
occur as multiple (more than 2) alleles in a genome
2.
What is the purpose of QTL mapping
a)
Calculation of the concordance values
b)
Identification of chromosomes regions
associated with complex traits
c)
Identification of additive genes
d)
All of the above
3.
Restriction fragment length polymorphisms
is
a)
Based on PCR
b)
Based on hybridization
c)
Both a and b
d)
None of the above
4.
Amplified fragment length polymorphisms is
a)
Based on PCR
b)
Based on hybridization
c)
Both a and b
d)
None of the above
5.
SCoT
is
a)
Based on PCR
b)
Based on hybridization
c)
Both a and b
d)
None of the above
6.
Marker may be
a)
Biochemical
b)
Morphological
c)
Molecular
d)
All of the above
7.
FISH
is
a)
Molecular cytogenetic technique
b)
Molecular Marker technique
c)
Both a and b
d)
None of the above
8.
Molecular markers may be due to
a)
Base pair changes
b)
Insertions and deletions
c)
Variation in the number of tandem repeats
d)
All of the above
9.
DNA Marker application
a)
Seed testing
b)
BSA
c)
Comparative maps
d)
All of the above
10.
Primer is a
a)
Short sequence of oligonucleotides
b)
Short sequence of polynucleotide
c)
Both a and b
d)
None of the above
11.
Criteria for primer design
a)
Size of the amplicon
b)
G-C content
c)
3’ terminal properties
d)
All of the above
12.
Primer melting temperature directly
propositional to
a)
GC content
b)
AT content
c)
Both a and b
d)
None of the above
13.
Primer annealing temperature depend on
a)
Melting temperature
b)
GC content
c)
AT content
d)
All of the above
14.
Common problems in primer designing
a)
Self complementary
b)
Hetero complementary
c)
Both a and b
d)
None of the above
15.
Lower delta-G value means
a)
Higher the quantity of energy needed to
fully break DNA
b)
Less quantity of energy needed to fully
break DNA
c)
High temperature needed to fully break DNA
d)
Both a and c
16.
Reverse primer codes
a)
5’ to 3’
b)
3’ to 5’
c)
Both a and b
d)
None of the above
17.
Forward primer codes
a)
5’ to 3’
b)
3’ to 5’
c)
Both a and b
d)
None of the above
18.
OligoAnalyzer use to analyse
a)
Hairpin
b)
Self-dimer
c)
Hetero-dimer
d)
All
of the above
19.
Software for primer designing
a)
PRIMO
b)
RAW
Primer
c)
Primer
quest
d)
All
of the above
20.
Tools needed for QTL mapping
a)
Mapping population
b)
Linkage map
c)
Trait
measured on some quantitative scale
d)
All
of the above
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