GPB509 (1+1) Biotechnology for Crop Improvement

 

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Biotechnology for Crop Improvement GPB509 (1+1)
Practical Examination
2017-18

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1. Write the role of following chemicals
   a. EtBr
   
   

   b. TAE buffer
   
   

   c. Agarose
   
   

   d. CTAB
   
   

   e. NaCl
   
   

   f. SDS
   
   

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2. Fill the blanks
   a. The extension step in PCR for SSR marker is at 72°C temperature.
   b. Annealing temperature for RAPD marker in PCR is at 36°C temperature.
   c. NTSYS is used for genomic sequencing.
   d. The denaturation step for RAPD markers is at 94°C temperature.

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3. Write overview of DNA isolation in crop plants.
   (1.0)

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4. What is gradient PCR?
   (1.0)

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5. Write utility of biophotometer.
   (1.0)

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6. Write note on Horizontal gel electrophoresis.
   (2.0)