PATH-604 Molecular Basis of Host Pathogen Interaction (Question Paper)

University of Agricultural Sciences, Raichur
College of Agriculture, Raichur
Final Examination

Course Title: Molecular Basis of Host Pathogen Interaction (PATH-604)
Credit: 2+1
Duration: 90 Min.
Total Marks: 35

---

A. Describe/write contribution (of scientist/s listed) in few words (any fifteen) (1×15=15)

1. PAMP and MAMP

2. Pathogenicity

3. Any two applications of genome editing in disease management

4. Avr genes

5. PTI and ETI

6. Defense genes

7. Virulence genes

8. Hypersensitive response

9. IC-PCR and RT-PCR

10. CRISPR

11. PR proteins

12. hrp gene cluster

13. B.J. Staskawicz

14. Stakman et al.

15. H.H. Flor

16. PRR

17. Cloning vectors

---

B. Answer the following questions (any five) (4×5=20)

1. Write classification of R genes and how R genes confirm resistance?

2. Write different pathogenicity genes in fungal and bacterial pathogens.

3. Explain the molecular basis for “Gene for Gene Hypothesis” by taking the example of two host genes (R1R2/r1r2) and two pathogen genes (A1A2/a1a2).

4. Write the different secretion systems present in pathogenic bacteria with examples. Describe the role of Type III secretion system.

5. Describe different reagents and their function in DNA isolation. What are the basic steps involved in the extraction of DNA from fungal mycelium?

6. What are the different techniques to assess the quality and quantity of DNA?