GPB 509 Biotechnology for Crop Improvement (1+1)

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Biotechnology for Crop Improvement GPB509 (1+1)
Final Examination 2017-18

Name:
ID No:
Marks: 35

1. Fill the blanks (5.0)
   a. QTL term coined by -------------------------
   b. NILs are very much useful in ---------------------- studies.
   c. Elements of biosensors are ------------------- and --------------------.
   d. Gramine is database for -----------------------------.
   e. MAPMAKER is used for -----------------------------.
   f. The mapping functions are ------------------ and --------------.
   g. Replicates mediated resistance is against ------------ organism.
   h. Biparental population has ----------------- no. of parents.
   i. Gene tagging without mapping is through ---------------------- method.

2. Say True or False (3.0)

   1. F2 population is homozygous and heterogeneous.

   2. Segregation ratio of codominant marker in RIL population is 1:1.

   3. The R² tells about phenotypic variance explained.

   4. MAGIC population has more than 100 lines as parents.

   5. QTL is Quantitative Trait Loci.

   6. Biosensor has physical and biological component.

3. Define the following (10.0)
   a. Somaclonal variation
   b. Protoplast
   c. Saturated Map
   d. Somatic Hybridization
   e. Cybrid
   f. Biosensors
   g. Microinjection
   h. RNAi
   i. Cryopreservation
   j. Micropropagation

4. Write advantages and disadvantages of F2 population (2.0)

5. Compare RIL population with DH population (2.0)

6. Write about MAB for gene pyramiding (2.0)

7. What are the requirements of QTL mapping (2.0)

8. Mention different statistical methods used for QTL detection (1.0)

9. Write about accelerated breeding (1.0)

10. Write about Agrobacterium mediated transformation method (2.0)

11. Write note on particle bombardment method (2.0)

12. Write applications of rDNA technology (2.0)