GPB 509 Biotechnology for Crop Improvement Question Paper

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Question paper for qualifying examination of Sr. MSc(Ag) Students
GPB509 2016-17
Total Marks: 5.25

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1. Expand the following (0.5 x 4 = 2.0)
   a. SNP: Single Nucleotide Polymorphism
   b. DAF: Diversity array Technology
   c. MAGIC: Multiparent Advanced Generation Intercross
   d. NCBI: National Centre for Biotechnology Information

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2. Fill in the blanks (0.5 x 4 = 2.0)
   a. Biophotometer is used for ________________ quantification of DNA while thermal cycler is used for ________________ amplification of DNA.
   b. MAPMAKER is for ____________ genetic map construction and NTSYS is for _____________ analysis.

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3. Define the following (0.25 x 4 = 1.0)
   a. Genetic map
   b. Physical map
   c. Allele Mining
   d. Dedifferentiation

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4. Explain the following (1.25)
   a. Compare traditional breeding and Marker Assisted Selection

   Genetic Map: Map representing the genes or QTLs (quantitative trait loci) markers on the genomes and the distance is measured based on centimorgan differences.
   Physical Map: A map representing the linkage groups, etc. of a genome and is expressed in terms of base-pair unit, which could be done by the presence/absence of markers based on the trait observation.

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