GPB509 Biotechnology for Crop Improvement (1+1)

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Biotechnology for Crop Improvement GPB509 (1+1)
Midterm Examination 2015-16

Marks: 20

Name: ___________
ID No: ___________

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1. Expand the following:

a. RAPD: ________________
b. SAMPL: ________________
c. SSR: ________________
d. DGGE: ________________
e. AFLP: ________________
f. DArT: ________________
(3.0)

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2. Fill the blanks:

a. Segregation ratio of RAPD marker in F2 population is _____________.
b. Segregation ratio of SSR marker in RIL population is _____________.
c. ____________ marker is suitable for whole genome fingerprinting.
d. ____________ markers are suitable for hybrid purity analysis.
e. ____________ marker referred as PCR-RFLP.
f. Southern hybridization is followed in ____________ marker.

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3. Compare primers of SSR and ISSR. (1.0)
4. Compare STS and EST markers. (2.0)
5. Reliability of RAPD marker is low. Give reasons. (1.0)
6. Write about SCAR marker. (1.0)
7. Explain SNP markers. Mention advantages, disadvantages and applications. (2.0)
8. Compare RAPD, AFLP, SSR and ISSR marker. (2.0)
9. Compare morphological and molecular markers. (2.0)
10. What are biochemical markers. Explain Isozyme and Allozyme markers. (3.0)