MBB 501 Principles of Biotechnology Question Paper

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Final Theory Paper
Principles of Biotechnology (MBB 501)
Date: 28/05/2021
Roll No.: ____________________
Discipline: ___________________
Time: 2.00 hrs

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PART – A (25 Marks)

1. Write ‘True’ or ‘False’ (12 Marks)
i) VirE2 protein of Agrobacterium helps in nuclear import of T-DNA
ii) In pCAMBIA binary vector, the first digit indicates plant selection marker
iii) CAPS marker can be used in map-based cloning of gene
iv) SSR primer designing does not need prior sequence information
v) ISSR fragment length variation is due to variation in number of microsatellite repeat units
vi) Nopaline is a modified amino acid
vii) Fluorescence is due to close proximity of donor and acceptor fluorophores
viii) The expression cassette for plant selectable marker gene lies outside the T-DNA region
ix) Vir genes of Ti plasmid of Agrobacterium tumefaciens can mediate T-DNA transfer in trans
x) Glyphosate herbicide kills plants by inhibiting glutamine synthetase
xi) Zinc finger binds dsDNA by recognizing four nucleotide sequence
xii) Fluorescence polarization depends on molecular mass

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2. Write short notes (Any four) (2×4 = 8 Marks)
i) Bt technology for insect resistance
ii) Herbicide tolerant GM crops
iii) CAPS marker
iv) Fluorescence Polarization
v) Mass Spectrometry-based SNP allele detection
vi) Recombinant insulin

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3. Answer any one (5 Marks)
EITHER
Write in detail the mechanism of Agrobacterium T-DNA transfer.
OR
Describe allele discrimination techniques for SNP genotyping.

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PART – B (25 Marks)

1. Write ‘True’ or ‘False’ (10 Marks)
a) Chromosomal theory of heredity was given by Sutton and Boveri in 1903.
b) Pseudogene is a non-functional DNA sequence that is nearly identical to that of a functional gene
c) There are 12 chromosomes in Arabidopsis
d) Northern hybridization is used for gene expression analysis
e) Dissociation curve describes amplification specificity in qRT-PCR
f) DSN1 enzyme is used in TILLING experiment
g) RNA is spotted on blot in dot blot experiment
h) Pyrosequencing is the most accurate NGS technology available
i) Proteins have variable solubility
j) Post-translational modifications increase the number of letters in amino acid alphabets

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2. Write short notes (Any four) (2.5×4 = 10 Marks)
a) Model organisms for genomics study
b) Structural genomics
c) Loss-of-function and Gain-of-function mutants
d) Emulsion PCR
e) Proteome array

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3. Long Answer (5 Marks)
Enlist first, second (next), and third generation sequencing technologies.
What are the advantages of next generation sequencing technologies over Sanger sequencing?

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