MBB 502 Question Paper Question Paper

MBB 502-2020

Final Term Examination
Date: 21st May 2021
Time: 3 hours
Maximum Marks: 60

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Instructions:

• Attempt all questions.

• All questions carry equal marks (10 marks each).

• Write answers to Part A, B, C, and D on separate sheets.

• Send each part as a separate PDF to the respective instructor’s email as mentioned below.

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PART A

Q1. Explain the following with diagrams: (5 marks each)

a) Mechanism of Attenuation in regulation of trp operon
b) Positive control of CAP protein in regulation of lac operon

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Q2. Write short notes on the following with diagrams: (5 marks each)

a) Ribosome binding assay in the elucidation of genetic code
b) Translocation step of prokaryotic translation

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PART B

Q1. Answer the following: (5 marks each)

a) Draw a schematic diagram showing different types of alternative splicing events in eukaryotic mRNA
b) Describe how viruses hijack host cell translational machinery

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Q2. Answer the following: (2 + 3 + 5 marks)

a) What is the role of eIF3 and eIF6 in translation? (2 marks)
b) Define hybrid state and classical state in translation (3 marks)
c) Describe regulation of translation initiation in eukaryotes (5 marks)

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PART C

Q1. Write notes on any four of the following: (2.5 marks each)

a) Reverse Transcriptase Enzyme
b) List the enzymes used in prokaryotic DNA replication
c) DNA Topoisomerase and its types
d) DNA Polymerase and the families
e) DNA Ligase enzyme and the two cofactors used

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PART D

Q1. Answer the following: (5 marks each)

1. What is DNA recombination? Describe the key steps involved in homologous recombination with appropriate diagram.

2. Define mutation and mention different types of gene mutation. Explain frameshift mutation with suitable diagram.

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