GPB 509 (1+1) Biotechnology for Crop Improvement

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Biotechnology for Crop Improvement GPB3509 (1+1)
2012-13

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1. Fill the blanks (1.0)
i) AP-PCR: Arbitrary Primer Polymerase Chain Reaction
ii) TGGE is: Thermal Gradient Gel Electrophoresis
iii) MAPMAKER is used for: Map Construction & Gene Mapping
iv) DART is: Diversity Assay Technology

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2. Define (2.0)
i) LOD: Limit of Detection
ii) Genetic Map: A diagrammatic representation of the arrangement of genes on a chromosome
iii) QTL: Quantitative Trait Loci
iv) Gene Pyramiding: A technique for stacking multiple genes in a plant to enhance its traits

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3. Explain about SSCP marker (2.0)

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4. What are SNPs? Write advantages, disadvantages, and applications. (2.0)

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5. Compare RFLP, AFLP and ISSR markers. (3.0)

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6. Write about SCAR marker and how it is different from RAPD. (3.0)

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7. What are utilities of markers in hybrid breeding? Discuss (3.0)

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8. Write schematic diagram to obtain different kinds of mapping populations and also mention genetic constitution and merits and demerits of,
i) F2s
ii) RILs and
iii) DH population
(3.0)

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9. Compare biochemical and morphological marker (1.0)

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10. What are InDels and EST database (1.0)