GPB 521 (2+0) Molecular Breeding PYQ (Question Paper)

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Molecular Breeding GPB 521 (2+0)
Announced Quiz 2017-18
Marks: 15

I. Fill the blanks (5.0)

1. Phosphoramidites are useful in ____________ synthesis.

2. Standard genes has GC content of ____________.

3. In silico candidate gene approach is also known as ____________ gene approach.

4. House keeping genes are controlled by ____________ promoters.

5. ____________ promoters depend on external stimuli.

6. QTL mapping is ____________ DNA fragments can be incorporated into Cosmids.

7. Gene knockout is ____________ genetics strategy.

8. ____________ is forward genetics strategy.

9. PUE is ____________ genetics strategy.

10. ____________ DNA fragments can be incorporated into Bacteriophages.

II. Define (5.0)

1. Candidate Gene

2. Forward genetics

3. Reverse genetics

4. Gene isolation

5. Restriction Enzymes

III. Answer the following (5.0)

1. Compare position dependent and function dependent strategy of candidate gene identification. (2.0)

2. Write about gene isolation based on protein sequence. (1.0)

3. Write steps in gene cloning. (1.0)

4. Write characteristics of ideal vector. (1.0)