UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Biotechnology for Crop Improvement GPB509 (1+1)
Midterm Examination 2017-18
Marks: 20

1. Expand the following (3.0)

  1. SSCP - Single Strand Conformational Polymorphism

  2. EST - Expressed Sequence Tags

  3. RAPD - Random Amplified Polymorphic DNA

  4. TGGE - Thermal Gradient Gel Electrophoresis

  5. AFLP - Amplified Fragment Length Polymorphism

  6. DArT - Diversity Array Technology

2. Fill the blanks (3.0)

  1. The length of SCAR primers is normally ____________ bp.

  2. The amplified product of SSR marker consists of ____________ polymorphism.

  3. Pyrosequencing is useful in ____________ marker analysis.

  4. DArT markers are suitable for hybrid purity analysis in ____________ crops.

  5. PCR-RFLP is known as ____________.

3. Answer the following (14.0)

  1. Compare primers of RAPD and ISSR. (1.0)

  2. Explain about SSR marker. (2.0)

  3. Write about SNP marker. (1.0)

  4. Write characters of ideal molecular marker. (2.0)

  5. Explain RFLP marker. Mention advantages, disadvantages and applications. (1.0)

  6. AFLP suitable for whole genome fingerprinting. Give reasons. (2.0)

  7. Write advantages, disadvantages and applications of STS marker. (2.0)

  8. Explain Isozyme and allozyme markers. (2.0)