GPB611 (2+1) Marker Assisted Selection for Crop Improvement

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Marker Assisted Selection for Crop Improvement GPB611 (2+1)
Midterm Examination 2019-20
Marks: 20

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1. Fill in the blanks:

a. The length of primers in SSR Markers is 20-24 bp
b. The expanded form of PAGE is Polyacrylamide Gel Electrophoresis
c. The expanded form of FRET is Fluorescence Resonance Energy Transfer
d. The gene tagging without mapping is by Bulk segregant analysis

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2. Write ratio for different population and marker.

a. F₁ population and dominant marker – 1:2:1 (genotype), 3:1 (phenotype)
b. RIL population with dominant marker – 1:1
c. RIL population with codominant marker – 1:1
d. DH population with codominant marker – 1:1

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3. Answer the following:

• Write the procedure of AFLP marker. (2.0)
• How to generate NIL and DH population? (2.0)
• What is the principle of SSR markers? (1.0)
• Compare STS and EST markers. (1.0)
• Write advantages and disadvantages of SSR marker. (2.0)
• How to obtain immortal F₂ population. (2.0)
• Write about SNP detection methods. (3.0)
• Write about BSA. (1.0)
• Write about DArT marker. (1.0)