GPB611 (2+1)Marker Assisted Selection for Crop Improvement

 

UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
College of Agriculture, Raichur
Department of Genetics and Plant Breeding
Marker Assisted Selection for Crop Improvement GPB611 (2+1)
Assessment/Quiz 2019-20
Marks: 15

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1. Expand the following: (3.0 Marks)

• CAPS – Cleaved Amplified Polymorphic Sequences
• SCAR – Sequence Characterized Amplified Region
• HDP-PCR – Hot Denaturation Polymerase Chain Reaction
• STR – Short Tandem Repeat
• SAMPL – Selective Amplification of Microsatellite Polymorphic Loci
• SSCP – Single-Strand Conformational Polymorphism

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2. Fill in the blanks: (3.0 Marks)

• EcoRI has a _______ bp recognition sequence.
• Alpha-amylase isozymes are analyzed by using Starch Gel Electrophoresis
• Coomassie Brilliant Blue is used for staining during total protein analysis.
• DGGE is Denaturing Gradient Gel Electrophoresis
• TGGE is Thermal Gradient Gel Electrophoresis
• The GC content of primers is normally 40-60%

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3. Define the following: (2.0 Marks)

• Genomic probe
• Isozyme
• Dominant marker
• Southern Hybridization

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4. Short Answer Questions:

• Write the procedure to develop SCAR marker. (1.0)
• Write about ISSR marker and discuss how it is different from RAPD marker. (1.0)
• Mention characteristics of ideal molecular marker. (1.0)
• Write advantages and disadvantages of RFLP marker. (1.0)
• Compare biochemical and DNA markers. (1.0)
• Discuss relevance of dominant and codominant markers in crop genotyping. (1.0)