|
Feature |
AFLP |
RFLP |
RAPD |
|
Full
Name |
Amplified Fragment Length
Polymorphism |
Restriction Fragment Length
Polymorphism |
Random Amplified Polymorphic DNA |
|
Technique |
PCR-based |
Gel Electrophoresis |
PCR-based |
|
Enzyme
Requirement |
None |
Restriction Enzymes |
None |
|
Primers |
Specific Primers (usually 2) |
Primers designed to flank restriction
sites |
Random Primers |
|
Target
DNA |
Whole Genome |
Specific Loci |
Random Regions of the Genome |
|
Resolution |
High |
Medium |
Low |
|
Reproducibility |
High |
High |
Low |
|
Information
Content |
High |
High |
Low |
|
Sensitivity |
High |
Medium |
Low |
|
Cost |
Moderate to High |
Moderate to Low |
Low |
|
Applications |
Genetic Mapping, Diversity
Assessment, Genome Analysis |
Genetic Mapping, Identification of
Variants, Cloning |
Genetic Fingerprinting, Population
Genetics, Taxonomy |
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