The recurrent selection backcross (RSB) procedure is a breeding method used to introgress favorable alleles from a donor parent into the genetic background of a recurrent parent while simultaneously improving the recurrent parent's performance for a target trait. Here's a brief overview of the RSB procedure and its usefulness and limitations:

Procedure:

·        Initial Cross: The RSB procedure begins with a cross between the recurrent parent (RP), which typically possesses desirable agronomic traits but lacks certain target traits, and a donor parent (DP), which carries alleles for the target traits of interest.

·        Backcrossing: The F1 hybrids resulting from the initial cross are backcrossed to the recurrent parent. In each backcross generation, individuals with the target trait from the F1 hybrid are selected and crossed back to the recurrent parent.

·        Selection: Progeny from each backcross generation are evaluated for the target trait and other desired agronomic traits. Selection is based on phenotype, with emphasis on individuals exhibiting the target trait and retaining desirable traits of the recurrent parent.

·        Recurrent Selection: Selected individuals from each backcross generation are used as parents for the subsequent generation of backcrosses, leading to the recurrent selection of individuals with improved performance for the target trait and the genetic background of the recurrent parent.

·        Fixation: The RSB procedure continues for several backcross generations until the desired level of introgression of the target trait alleles from the donor parent into the genetic background of the recurrent parent is achieved. Selection may involve selfing or inbreeding to fix desired alleles and traits.

Usefulness:

·        Trait Introgression: RSB allows for the introgression of target trait alleles from a donor parent into the genetic background of the recurrent parent while retaining desirable traits of the recurrent parent.

·        Genetic Improvement: The RSB procedure facilitates the genetic improvement of the recurrent parent for specific target traits by systematically selecting and introgressing favorable alleles over multiple backcross generations.

·        Trait Stabilization: By repeatedly backcrossing and selecting for individuals with the target trait, the RSB procedure helps stabilize the expression of the trait in the genetic background of the recurrent parent.

·        Efficiency: RSB is relatively efficient and does not require extensive genotyping or marker-assisted selection compared to other breeding methods such as marker-assisted backcrossing.

Limitations:

·        Genetic Dilution: The introgression of target trait alleles from the donor parent may lead to genetic dilution or the loss of favorable alleles from the recurrent parent's genetic background, potentially reducing overall performance or adaptability.

·        Time and Resource Intensive: RSB requires multiple backcross generations to achieve desired trait introgression and genetic stabilization, making it a time-consuming and resource-intensive breeding method.

·        Inbreeding Depression: The repeated backcrossing and selection process can lead to inbreeding depression if not carefully managed, potentially reducing vigor and fitness in the resulting breeding population.

·        Dependence on Recurrent Parent: The success of RSB depends on the genetic diversity and performance of the recurrent parent, and introgression may be limited by the genetic distance between the recurrent and donor parents.

In summary, the recurrent selection backcross procedure is a useful breeding method for introgressing target traits into the genetic background of a recurrent parent while simultaneously improving its performance. However, it has limitations such as genetic dilution, time and resource intensity, risk of inbreeding depression, and dependence on the recurrent parent's genetic diversity and performance. Careful management and selection are essential to optimize the effectiveness of the RSB procedure in genetic improvement programs.