GP502 - (Principles of Cytogenetics) Question Paper

Course: GP502 (Principles of Cytogenetics)
Final Examination (40 Marks)
(All the questions are compulsory)
Date: 25-05-2021
Time: 10:00 am

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Fill in the blanks (1 mark each)

1. Triticum zhukovskyii is an _______________ species.

2. Autopolyploids follow _________________ inheritance.

3. Best source of trisomics is _____________.

4. Colchicine is a cell poison alkaloid extracted from Colchicum autumnale which affects ___________ during cell cycle.

5. The first alien gene to be transferred from Ae. umbellulata to wheat is ________ imparting resistance to leaf rust fungus.

6. An _____________ is an abnormal chromosome having two identical arms.

7. The failure of chromosomes or chromatids to disjoin and move to opposite poles during cell division is known as ______________.

8. Allopolyploids behaving like diploids are termed as ____________.

9. Synaptonemal complex is formed during __________ substage of Prophase I.

10. Modern day seedless banana cultivars are ____________ in nature.

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Write short notes on: (2 marks each)

11. Robertsonian translocation

12. Bar phenotype in Drosophila

13. Semigamy and its role in cotton breeding

14. Doubled haploids (DH)

15. Yeast artificial chromosome - structure and utility

16. Dicentric bridge

17. Compensatory trisomics

18. Apomixis and two different models to explain apomixis

19. Bulbosum technique in barley

20. Chiasmata theory

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Compare and contrast (2 marks each)

21. Pericentric and paracentric inversions

22. Alternate and adjacent segregation

23. Autopolyploidy and allopolyploidy

24. Breeding behavior of trisomics and monosomics

25. Holiday’s Model and Single strand break model

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Practical Examination (20 marks)

1. What is meant by karyotyping? What is the difference between karyotype and ideogram? (2 marks)

2. What are the banding techniques used for karyotyping? (2 marks)

3. Write short notes on FISH (2 marks)

4. How Feulgen stain is prepared? (2 marks)

5. Differentiate between magnification and resolution. (2 marks)

6. Enlist the stages of Meiosis I along with changes happening at each stage. (5 marks)

7. Briefly explain how to prepare mitotic (root cell) slide. (5 marks)