Marker Assisted Selection for Crop Improvement GPB611(2+1)

PART A Fill in the blanks

i) SSCP is single stranded conformation polymorphism (0.5)
ii) DAF is DNA amplification finger printing (0.5)

i) SCAR is sequence characterized amplified nylon (0.5)
ii) CAPS marker is also known as cleaved amplified polymorphism sequence (0.5)

i) Gene tagging without mapping is through Bulk Segregant analysis (0.5)
ii) Kaldane and Kosambi are mapping functions (0.5)

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II. Define the following: (1 x 5 = 5)

1. Linkage Disequilibrium

2. Gene pyramiding

3. GWAS

4. Polymorphism

5. Functional markers

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PART B Answer the following

i) How the molecular markers are useful in diversity analysis. (2.0)
ii) Why reproducibility of RAPD is low? Give reasons. (2.0)

i) Brief the AFLP technique. (2.0)
ii) Compare STS and EST markers. (2.0)

i) Write about limitations of MAS. (2.0)
ii) Compare F₂ and RIL population. (2.0)

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PART C Answer the following

i) How bioinformatics could be used in crop improvement. (5.0)
ii) Write about Map based cloning with an example. (5.0)

i) Write about MAGIC population. Discuss its merits and demerits. (5.0)
ii) Write the procedure for QTL mapping in crop plants. (5.0)

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