Marker Development and Gene Mapping ( Question Paper )

 

Substantiate the following with suitable illustrations and examples

·        DH and NILs are excellent resources in mapping and fine mapping genomic regions controlling target traits, respectively.

·        Nucleotide sequences (on both strands of DNA double helix) flanking repeat motifs serve as information for primer designing and SSR marker development.

·        Use of most appropriate separation system (SSR amplicons/alleles) is critical during SSR marker assay.

·        SSR markers are efficient and effective tools that aid present generation of plant breeders right from initiation till completion of successful plant breeding program(s).

·        RFLP markers serve as effective resources in physical map construction.

·        Genomic and c DNA libraries are integral part of marker development efforts.

·        Suitable DNA marker based classification of F₂ enables effective estimation of marker effects and QTL detection.

·        Ambiguous and dominant marker systems are not effective and efficient in linkage mapping and diversity analysis.

·        Two individuals contrasting at the phenotypic level need not exhibit polymorphism at the marker loci. Similarly, two individuals differentiated by marker loci need not necessarily be contrasting for the target trait.

·        Most often there is no agreement between physical and recombination based genetic maps.