GPB MARKER DEVELOPMENT AND GENE MAPPING ( (Question Paper)

 

·        What is the principle of assigning markers to different linkage groups/chromosomes

·        What is the basic genetic principle of DNA marker-based linkage map development

·        How may marker orders be possible with ‘n’ number of markers

·        Who coined the term quantitative trait loci (QTL)?

·        What is the formula for estimating recombination between marker loci in RIL population

·        How do you classify QTL mapping methods based on number of markers used per analysis?

·        What are the criteria to select the parents for developing mapping populations

·        What is the principle detecting QTL using bulk segregating analysis

·        Mention the strategy to phenotype F₂ mapping population

·        Mention number of marker classes possible in F₂, backcross, recombinant inbred lines (RIL) and di-haploid populations

·        Define mapping function and mention their types

·        Mention the type of markers to be used to genotype F₂, backcross, recombinant inbred lines (RIL), di-haploid mapping population and why?

·        What is the principle of detecting QTL in single marker analysis and illustrate with a hypothetical numerical example