Feature

AFLP

RFLP

RAPD

Full Name

Amplified Fragment Length Polymorphism

Restriction Fragment Length Polymorphism

Random Amplified Polymorphic DNA

Technique

PCR-based

Gel Electrophoresis

PCR-based

Enzyme Requirement

None

Restriction Enzymes

None

Primers

Specific Primers (usually 2)

Primers designed to flank restriction sites

Random Primers

Target DNA

Whole Genome

Specific Loci

Random Regions of the Genome

Resolution

High

Medium

Low

Reproducibility

High

High

Low

Information Content

High

High

Low

Sensitivity

High

Medium

Low

Cost

Moderate to High

Moderate to Low

Low

Applications

Genetic Mapping, Diversity Assessment, Genome Analysis

Genetic Mapping, Identification of Variants, Cloning

Genetic Fingerprinting, Population Genetics, Taxonomy