What is the main purpose of using a cover slip in microscopy?
A) To increase the light intensity
B) To protect the objective lens
C) To flatten the specimen and prevent dehydration
D) To magnify the specimen further
In fluorescence microscopy, what does the term "excitation wavelength" refer to?
A) The wavelength of light emitted by the fluorophore
B) The wavelength of light absorbed by the fluorophore
C) The wavelength of light used to illuminate the specimen
D) The wavelength of light filtered by the microscope
Which type of microscope is best suited for observing living, unstained cells?
A) Phase Contrast Microscope
B) Scanning Electron Microscope
C) Transmission Electron Microscope
D) Confocal Microscope
What is "resolution limit" in microscopy?
A) The maximum size of the specimen that can be observed
B) The minimum distance between two points that can still be distinguished as separate
C) The maximum magnification achievable by the microscope
D) The depth of field of the microscope
Which microscopy technique uses a scanning probe to examine the surface of specimens?
A) Atomic Force Microscopy (AFM)
B) Confocal Microscopy
C) Brightfield Microscopy
D) Phase Contrast Microscopy
What is the main limitation of light microscopy compared to electron microscopy?
A) Light microscopy cannot achieve high enough magnification
B) Light microscopy requires specimens to be coated with metal
C) Light microscopy provides images in three dimensions
D) Light microscopy can only observe live specimens
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Which of the following techniques is used to measure the thickness of a specimen in a microscope?
A) Depth of Field Measurement
B) Numerical Aperture Calculation
C) Magnification Factor
D) Resolution Limit
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Question 34: What is the role of the eyepiece in a microscope?
A) To focus light onto the specimen
B) To magnify the image for the observer
C) To adjust the light intensity
D) To hold the specimen in place
Which microscopy technique is best for visualizing molecular interactions within cells?
A) Fluorescence Microscopy
B) Brightfield Microscopy
C) Scanning Electron Microscopy
D) Transmission Electron Microscopy
In which type of microscopy is a specimen typically stained with heavy metals?
A) Transmission Electron Microscopy (TEM)
B) Scanning Electron Microscopy (SEM)
C) Fluorescence Microscopy
D) Phase Contrast Microscopy
What is a "stage micrometer" used for in microscopy?
A) To calibrate the light intensity
B) To measure the size of specimens
C) To adjust the focusing mechanism
D) To calibrate the objective lenses
What is the key feature of a "dual-beam" scanning electron microscope (SEM)?
A) It uses two electron beams to provide high-resolution images
B) It simultaneously captures images of the specimen from two angles
C) It allows for the imaging of both surface and internal structures
D) It provides three-dimensional imaging using two detectors
What is the main advantage of using a "cold light source" in microscopy?
A) It increases image resolution
B) It reduces specimen heating
C) It provides high contrast images
D) It enhances fluorescence
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