181) Which of the following blotting methods is used for protein?
A) Southern
B) Northern
C) Western
D) Eastern
182) The technique used to separate DNA fragments is?
A) Southern blotting
B) Gel electrophoresis
C) SSR
D) PCR
183) Transfer of nucleic acid fragment from gel to nitrocellulose or nylon membrane is known as?
A) Labelling
B) Probing
C) Blotting
D) Hybridization
184) A procedure in which RNA fragments are transferred from an agarose gel to nitrocellulose membranes or DBM membrane is known as?
A) Northern blotting
B) Southern blotting
C) Western blotting
D) Both A and B
185) Invitro amplification of DNA is done by?
A) Polymerase Chain Reaction
B) Polymerase Chain Reduction
C) Gel documentation system
D) Gel electrophoresis
186) Annealing step of PCR involves?
A) Separation of two strands
B) Rearrangement of DNA
C) Joining of primer to complementary strand
D) Super coiling
187) Extension step of PCR occurs at which temperature?
A) 90 oC
B) 72 oC
C) 60 oC
D) 94 oC
188) Which of the following is not used for DNA fingerprinting?
A) RAPD
B) RFLP
C) SSR
D) Blotting
189) Short single stranded oligonucleotide that is used for DNA amplification in PCR is?
A) cDNA
B) Primer
C) QTL
D) Probe
190) The technique used to separate DNA fragments is?
A) Southern blotting
B) Gel electrophoresis
C) SSR
D) PCR

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